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. 2017 Apr 15;28(8):1111–1122. doi: 10.1091/mbc.E16-07-0499

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© 2017 Iaea et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Expression of omega-1 loop mutant (W108D) of STARD4 does not restore sterol transport. (A) U2OS-SRA cells were transfected with either wild-type or W108D mCherry-FKBP-STARD4 48 h before the experiment. STARD4 CRISPR constructs were transfected for 18 h before the experiment. FRAP measurements for cells expressing wild-type or W108D mCherry-FKBP-STARD4 in cells expressing STARD4 CRISPR constructs. Control, t_1/2 = 14.5 ± 1.5 min; SD4 CRISPR + SD4, t_1/2 = 11.0 ± 1.6 min; SD4 CRISPR + W108D SD4, t_1/2 = 21.9 ± 1.5 min. (B) DHE efflux curves for cells expressing wild-type or W108D mCherry-FKBP-STARD4 in cells expressing STARD4 CRISPR constructs. Control, t_1/2 = 14.9 ± 0.9 min; SD4 CRISPR + SD4, t_1/2 = 12.5 ± 0.4 min; SD4 CRISPR + W108D SD4, t_1/2 = 19.0 ± 0.2 min. Each data point is derived from an average of at least 10 experiments (±SE). Data are fitted to single-exponential curves.