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. 2017 Apr 15;28(8):1111–1122. doi: 10.1091/mbc.E16-07-0499

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© 2017 Iaea et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — STARD4 is a major nonvesicular sterol transport protein. U2OS-SRA cells were transfected with STARD4 CRISPR constructs and/or treated with energy depletion (ED) medium. (A) FRAP measurements for cells expressing STARD4 CRISPR constructs with or without ED. Control, t_1/2 = 14.5 ± 1.5 min; SD4 CRISPR, t_1/2 = 22.4 ± 2.1 min; ED, t_1/2 = 21.2 ± 3.2 min; SD4 CRISPR + ED, t_1/2 = 24.1 ± 1.3 min. (B) DHE efflux measurements for cells expressing STARD4 CRISPR constructs with or without ED. Control, t_1/2 = 14.9 ± 0.9 min; SD4 CRISPR, t_1/2 = 20.2 ± 0.4 min; ED, t_1/2 = 18.3 ± 1.1 min; SD4 CRISPR + ED, t_1/2 = 24.8 ± 0.3 min. (C) Normalized sterol transfer rate constants for FRAP (red) and efflux (blue) experiments under control, ED, STARD4-CRISPR and CRISPR-ED conditions. Each data point is derived from an average of at least nine experiments (±SE). Data are fitted to single-exponential curves.