FIGURE 2:

Truncation of Chp1 in recently branched species of Schizosaccharomyces. (A) Schematic representation of the structure of complex of Chp1 with Tas3 (adapted from Debeauchamp et al., 2008). The N-terminal (residues 9–83) of Tas3 attaches to the C-terminal of Chp1 (residues 504–960). Model shows interaction of Chp1 (C-terminal SPOC, DOMII, and PIN domains) with Tas3 (N-terminal domain), which is crucial for centromeric silencing by H3K9 methylation. (B) Comparison of domain structure of Chp1 of S. japonicus, S. pombe, S. octosporus, and S. cryophilus, showing loss of its C-terminal (which interacts with the N-terminal of Tas3) in S. octosporus and S. cryophilus. Also indicated is the domain structure of Tas3 of S. pombe.