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. 2017 Apr 15;28(8):1147–1159. doi: 10.1091/mbc.E16-09-0632

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© 2017 Borze˛cka-Solarz et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Mutations of specific domains of Lyn affect its influence on TNF-α and CCL5/RANTES production in murine peritoneal macrophages. Cells nucleofected with indicated Lyn constructs or GFP alone were analyzed by immunoblotting with anti-Lyn antibody (A) or stimulated with 100 ng/ml LPS to measure the amount of released TNF-α and CCL5/RANTES by ELISA (B, C). In A, arrowhead points to endogenous Lyn. In B and C, results are mean ± SD from three experiments run in triplicate. *Significantly different from LPS-stimulated cells transfected with GFP alone at p ≤ 0.05.