FIGURE 1:

Detection of a peptide-degrading activity in cym1Δ mitochondria. (A) Degradation of Cox4^1–17 (lanes 1–8) and Sod2^1–18 (lanes 9–16) presequence peptides in soluble extracts of mitochondria from wild-type (WT) and cym1Δ yeast strains. Samples were analyzed by SDS–PAGE and immunodecoration. Aco1 and Mas1 served as loading controls. (B) Degradation of Cox4^1–17 presequence peptide in mitochondrial extract from cym1Δ strain in the presence or absence of protease inhibitors (5 mM EDTA and 1 mM o-phenanthroline [o-Phe] or 1 mM PMSF). Ssc1, loading control.