Figure 2. Loss of jumu causes expansion of the populations of Col+ PSC cells in the lymph gland.
(A–E) Immunostaining against the PSC cell marker Antp (green) shows an increased number and ectopic expansion of PSC cells in jumu-mutant lymph glands. (F–G’) Increased hhF4f-GFP-positive PSC cells (green) are located throughout the lymph glands of jumuDf2.12 third-instar larvae, and these cells are co-localized with Antp (red). (H–I’) Antp immunostaining (red) of the lymph glands of third-instar larvae expressing GFP under the control of col-Gal4 (green) shows col>GFP ectopic expression and co-localization with Antp in jumuDf2.12. (J–L) Immunostaining against Col (green) shows ectopic expansion of Col+ PSC cells in jumu-mutant lymph glands. (M–P) Deficiency in the col levels (col−/+) reduces the numbers of both Col+ and Antp+ PSC cells in jumuDf2.12. (Q–T) Deficiency in the col levels (col−/+) reduces Shg expression (green) (Q, R) and increases the differentiation of plasmatocytes (red) (S, T) in jumuDf2.12. (U) Quantification of the number of PSC cells (Antp+ cells) per lymph gland lobe. The data are presented through a box-and-whisker representation. **p<0.01 and ***p<0.001 (one-way ANOVA). Dashed white and yellow lines outline the edges of the primary and secondary lobes, respectively, in E and L. Scale bars: 100 μm.