Figure 3. jumu is required for the prevention of ectopic PSC cells derived from the PSC domain during larval development.

(A–D) Immunostaining against the PSC cell marker Antp (green) in L1 larvae (A, B) and L2 larvae (C, D). (E–J’) The expanded Antp⁺ PSC cells (red) in jumu^Df2.12 lymph glands show no co-localization with dome-GFP⁺ cells during the L1 larvae (E–F’), L2 larvae (G–H’) and early L3 (I–J’) stages. (K–L’) The expanded Antp⁺ PSC cells (red) in jumu^Df2.12 lymph glands show no co-localization with Hml-GFP⁺ cells during the early L3 (K, K’) and L3 (L, L’) stages. Scale bars: 50 μm.

DOI: http://dx.doi.org/10.7554/eLife.25094.004