Figure 2.

Inhibitory effects of triamterene on TGR5 agonist-induced calcium influx in NCI-H716 cells.

Notes: LCA induced dose-dependent increases in cellular calcium (Ca²⁺) levels in NCI-H716 cells that were markedly inhibited by triamterene, as shown in (A). Similar effects of triamterene on betulinic acid-induced Ca²⁺ mobilization are shown in (B). Values (means ± SE) were obtained from eight determinations in each group. *P<0.05 and **P<0.01 compared with the vehicle-treated group at the same concentration.

Abbreviations: LCA, lithocholic acid; SE, standard error; TGR5, Takeda G-protein-coupled receptor 5.