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. Author manuscript; available in PMC: 2017 Oct 5.
Published in final edited form as: Nature. 2017 Apr 5;544(7649):185–190. doi: 10.1038/nature21686

Extended Data Figure 5. Delta-9 desaturases FAT-6 and FAT-7 and MUFA oleic acid mediate the longevity of H3K4me3-methyltransferase deficient worms.

Extended Data Figure 5

a, ORO quantification. Mean ± s.d., n ≥ 29 worms per condition. b, ash-2 RNAi leads to lifespan extension in control (P < 0.0001, log-rank), but not in fat-6(tm331);fat-7(wa36) mutants. c, ash-2 RNAi extends lifespan in both wild-type worms and fat-7(wa36) single mutants (P < 0.0001, log-rank). d, ash-2 RNAi extends lifespan in fat-6(tm331) single mutants (P = 0.0162, log-rank), but lifespan extension by ash-2 RNAi is reduced in fat-6(tm331) mutants (9.26%) compared to wild-type worms (20.46%) (P = 0.0072, two-way ANOVA). e, ash-2 RNAi extends lifespan in fat-5(tm420);fat-7(wa36) double mutants (P < 0.0001, log-rank). f, ash-2 RNAi extends lifespan in fat-5(tm420);fat-6(tm331) worms (P = 0.0002, log-rank), but lifespan extension by ash-2 RNAi is reduced in fat-5(tm420);fat-6(tm331) mutants (14.03%) compared to wild-type worms (20.46%) (P = 0.0358, two-way ANOVA). g-h, GC-MS quantification of oleic acid. Mean ± s.e.m. of 3 biological replicates. i, ORO quantification. Mean ± s.d., n ≥ 13 worms per condition. Boxed conditions are identical to Fig. 4g. j, Oleic acid supplementation extends lifespan (P < 0.0001, log-rank), which is not further extended by ash-2 RNAi. Oleic acid supplementation extends lifespan in fat-7;ash-2 RNAi worms (P < 0.0001, log-rank). Boxed conditions are identical to Fig. 4h. k, ORO quantification. Mean ± s.d., n ≥ 21 worms per condition. l, GC-MS quantification of MUFAs. Mean ± s.e.m. of 2 independent experiments, each with 2-3 biological replicates. m, RT-qPCR. Mean ± s.e.m. of 2 independent experiments, each with 3 biological replicates. n, ORO quantification. Mean ± s.d., n ≥ 25 worms per condition. o, ash-2 RNAi extends lifespan in control (P < 0.0001, log-rank) but not fat-2 RNAi worms. c, i-j, n-o, Representative of 2 experiments. P values: a, g-i, k, n, Kruskal-Wallis with Dunn's correction; l-m, Two-tailed Mann-Whitney with Benjamini-Hochberg correction. * P < 0.05, ** P < 0.01.