Skip to main content
. Author manuscript; available in PMC: 2017 Oct 5.
Published in final edited form as: Nature. 2017 Apr 5;544(7649):185–190. doi: 10.1038/nature21686

Figure 4. MUFA accumulation is critical for the longevity of H3K4me3-methyltransferase deficient worms.

Figure 4

a, Conserved transcriptional network regulating delta-9 desaturase genes. b, Images and quantification of SBP-1 nuclear accumulation. Mean ± s.d. of 2 independent experiments, each with n = 4-6 nuclei per worm of ≥ 8 worms per condition. c, RT-qPCR. Mean ± s.e.m. of 3 independent experiments, each with 3 biological replicates. d, GC-MS. Mean ± s.e.m. of 2 independent experiments, each with 2-3 biological replicates. e, ORO quantification. Mean ± s.d., n ≥ 32 worms per condition. f, Lifespan extension by ash-2 RNAi is reduced in sbp-1 RNAi (9.78%) compared to control worms (21.90%) (P = 0.0003, two-way ANOVA). g, As in e, n ≥ 13 worms per condition. h, Dietary oleic acid extends lifespan in the context of fat-7;ash-2 double RNAi (P < 0.0001, log-rank). g, h, Representative of 2 experiments. P values: b, Two-tailed Mann-Whitney; e, g, Kruskal-Wallis with Dunn's correction; c, d, Two-tailed Mann-Whitney with Benjamini-Hochberg correction. * P < 0.05, ** P < 0.01.