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. Author manuscript; available in PMC: 2017 Oct 5.
Published in final edited form as: Nature. 2017 Apr 5;544(7649):185–190. doi: 10.1038/nature21686

Extended Data Figure 1. Deficiency in H3K4me3 modifiers leads to fat accumulation in the intestine without impacting fertility.

Extended Data Figure 1

a, ORO quantification. Mean ± s.d., n ≥ 17 worms per condition. b, ORO images. Scale bar: 100 μm. c, ORO quantification. Mean ± s.d., n ≥ 9 dissected intestines per condition. d-e, Nile Red staining and quantification. Mean ± s.d., n ≥ 7 dissected intestines (d) or n ≥ 11 worms (e) per condition. Scale bar: 100 μm. f, Autofluorescence and quantification. Mean ± s.d., n ≥ 17 worms per condition. Scale bar: 100 μm. g, Fertility quantification of live brood size (i), fertilized eggs (ii), and unfertilized oocytes (iii) laid per worm. Mean ± s.d., n ≥ 25 worms per condition. h, RT-qPCR. Mean ± s.e.m. of 2 independent experiments, each with 2-3 biological replicates. i, RT-qPCR. Mean ± s.e.m. from 2 independent experiments, each with 2-3 biological replicates. j, ORO quantification. Mean ± s.d., n ≥ 26 worms per condition. k, ORO quantification. Mean ± s.d., n ≥ 29 worms per condition. l, RT-qPCR. Mean ± s.e.m. of 3 biological replicates. m, D.I.C. (Nomarski) and GFP fluorescence images. n, ORO quantification. Mean ± s.d., n ≥ 26 worms per condition. a-f, k (rescue line #1), n, Representative of 2 experiments. P values: a, c-g, Two-tailed Mann-Whitney; h-i, Two-tailed Mann-Whitney with Benjamini-Hochberg correction; j-k, n, Kruskal-Wallis with Dunn's correction; l, Two-tailed t-test with Benjamini-Hochberg correction. * P < 0.05, ** P < 0.01.