Skip to main content
. Author manuscript; available in PMC: 2017 Sep 20.
Published in final edited form as: Nat Chem Biol. 2017 Mar 20;13(5):551–557. doi: 10.1038/nchembio.2336

Fig. 2. Threonine dehydrogenase is required for VqmA activation.

Fig. 2

(A) Schematic of the V. cholerae kbl-tdh genomic locus. Black triangles indicate transposon insertion sites from a genome-wide screen for regulators of vqmR expression. (B) vqmR promoter activity was measured using PvqmR::mkate2 in wild-type and Δtdh V. cholerae strains carrying the indicated plasmids following growth for 10 h in LB and normalization of OD600. The pCtr. plasmid is the empty parent vector. Plasmids ptdh and pkbl produce Tdh and Kbl, respectively. Plasmids ptdh(C40A) and ptdh(H92R) produce catalytically inactive Tdh variants. Errors bars denote the standard deviation of three independent biological replicates.