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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Biol Psychiatry. 2016 Oct 13;81(11):959–970. doi: 10.1016/j.biopsych.2016.10.005

Figure 3. NAC glutamate correlates of MA addiction vulnerability/resiliency in an inbred murine model.

Figure 3

(A) Immunoblotting was employed on MA-induced CPP, CPA and Neutral-B6 mice to assay for indices of protein levels of glutamate transmission within NAC subregions [CPP Score: F(1,37)=121.00, p<0.0001; *p<0.05 vs. Neutral, LSD post-hoc tests; +p<0.05 vs. CPP Score = 0 sec, one-sample t-tests]. (B) Within the NACc, mGlu1 and mGlu5 levels were highest in CPP mice [for mGlu1: [F(3,49)=21.88, p<0.0001, ***p<0.05 vs. all other groups, LSD post-hoc tests; for mGlu5: [F(3,49)=2.70, p=0.06; for mGlu2/3, p=0.34] and the expression of both mGlu1 and mGlu5 was predicted by CPP Score (from panel a). (C) Homer2a/b expression was also elevated within the NACc of CPP mice [for Homer2a/b: F(3,49)=8.92, p<0.0001; ***p<0.05 vs. all other groups, LSD post-hoc tests; for Homer1b/c: p=0.93] with expression also predicted by CPP score. (D) Within the NACs, Homer2a/b levels were also the most highly expressed in CPP mice [F(3,41)=8.10, p<0.0001; *p<0.05 Neutral vs. SAL; ***p<0.05 CPP vs. other groups, LSD post-hoc tests]. Samples sizes are indicated in parentheses.