Fig. 3.

PsAKR1 expressing tobacco transgenic seeds showed improved seed vigour and reduced reactive carbonyl compounds, a Transgenic seeds showing more TTC staining than wild type seeds, b Extent of TTC reduction (an indication of seed viability in WT and transgenic seeds at different durations of ageing treatment, c Differential seed germination of WT and transgenic seeds, d Germination percentage of transgenic and wild type seeds after accelerated ageing treatments, error bar indicate mean values from three biological replicates that are having 10 seeds each. t-test for both wildtype and transgenic seeds were conducted. Alphabets with same letters are non-significant at p = < 0.05. e, f g, h and i Correlations between germination % with TTC, methylgloxal (MG) content, malondialdehyde (MDA) content, Amadori product and Maillard products. Data points in colour indicate different time points of wild type seeds exposed to aging conditions at control (red), 48 h (yellow) and 72 h (green)