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. 2017 Mar 14;23(2):471–475. doi: 10.1007/s12298-017-0425-z

Table 1.

Effect of duration of callus incubation on MS medium supplemented with different concentrations of 2,4 -D on somatic embryogenesis

Conc. of 2,4-D Duration of incubation in weeks
4 Weeks 8 Weeks 12 Weeks 16 Weeks 20 Weeks
Type of callus % of embryo genesis Type of callus % of embryo genesis Type of callus % of embryo genesis Type of callus % of embryo genesis Type of callus % of embryo genesis
1.0 Friable 0.00 ± 0.00 Sticky 0.00 ± 0.00b Compact 4.17 ± 0.17c Embryogenic 12.50 ± 1.22b Embryogenic 66.67 ± 8.33b
2.5 Compact sticky 0.00 ± 0.00 Compact
Embryogenic
25.00 ± 1.22a Embryogenic 87.50 ± 2.22a Embryogenic 100.00 ± 0.00a Embryogenic 100.00 ± 0.00a
5.0 Sticky 0.00 ± 0.00 Compact 0.00 ± 0.00b Embryogenic 37.50 ± 1.22b Embryogenic 75.00 ± 1.43a Embryogenic 100.00 ± 0.00a
10.0 Sticky 0.00 ± 0.00 Compact
Embryogenic
8.33 ± 0.17b Embryogenic 70.83 ± 1.17a Embryogenic 91.67 ± 2.33a Embryogenic 100.00 ± 0.00a
Treatment Df (n − 1) = 3 8.00** 39.33*** 19.00*** 16.00***

Means within a column followed by same letters are not significantly (p < 0.05) different as determined by Duncan’s Multiple Range test

NS non significant

*** F value is highly significant at p < 0.001 level; **significant at p < 0.01 level; *significant at p < 0.05