Table 1.
Conc. of 2,4-D | Duration of incubation in weeks | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
4 Weeks | 8 Weeks | 12 Weeks | 16 Weeks | 20 Weeks | ||||||
Type of callus | % of embryo genesis | Type of callus | % of embryo genesis | Type of callus | % of embryo genesis | Type of callus | % of embryo genesis | Type of callus | % of embryo genesis | |
1.0 | Friable | 0.00 ± 0.00 | Sticky | 0.00 ± 0.00b | Compact | 4.17 ± 0.17c | Embryogenic | 12.50 ± 1.22b | Embryogenic | 66.67 ± 8.33b |
2.5 | Compact sticky | 0.00 ± 0.00 | Compact Embryogenic |
25.00 ± 1.22a | Embryogenic | 87.50 ± 2.22a | Embryogenic | 100.00 ± 0.00a | Embryogenic | 100.00 ± 0.00a |
5.0 | Sticky | 0.00 ± 0.00 | Compact | 0.00 ± 0.00b | Embryogenic | 37.50 ± 1.22b | Embryogenic | 75.00 ± 1.43a | Embryogenic | 100.00 ± 0.00a |
10.0 | Sticky | 0.00 ± 0.00 | Compact Embryogenic |
8.33 ± 0.17b | Embryogenic | 70.83 ± 1.17a | Embryogenic | 91.67 ± 2.33a | Embryogenic | 100.00 ± 0.00a |
Treatment Df (n − 1) = 3 | – | – | 8.00** | – | 39.33*** | – | 19.00*** | – | 16.00*** |
Means within a column followed by same letters are not significantly (p < 0.05) different as determined by Duncan’s Multiple Range test
NS non significant
*** F value is highly significant at p < 0.001 level; **significant at p < 0.01 level; *significant at p < 0.05