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. 2017 Apr 13;5:22. doi: 10.1186/s40635-017-0135-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — The effects of hyperoxia on nitrotyrosine levels in hMVEC. Figure shows representative pictures displaying a nitrotyrosine signal after 24 h exposure to 20% O₂ (control) and localization of the signal under normal culture conditions, b nitrotyrosine signal after 24 h 50% O₂ exposure, and c nitrotyrosine signal after 24 h 95% O₂ exposure. d Mean fluorescence intensity (MFI) was calculated per cell, and data is expressed as mean ± SD; N = 3, all data non-significant (P > 0.05)