Fig. 2.

Quantitative analyses of PhCRT mRNA in Petunia WT (a), si1PhCRT (b), and scr_si1PhCRT (c) elongated pollen tubes. Both in WT and scr_si1PhCRT pollen tubes, PhCRT transcripts were diffusely distributed throughout the tube shank, extending from the base to the subapical zone (a, c). In contrast, si1PhCRT pollen tube was almost devoid of hybridization signals (b). d Negative FISH control. e The level of PhCRT transcripts was 85% lower in si1PhCRT pollen tubes compared to WT and scr_si1PhCRT tubes. Graphs show the relative PhCRT mRNA levels in pollen tubes elongating in different culture conditions (mean of 20 replicates for each experiment variant and standard deviation). Arbitral units on the y-axis show total FISH intensity (pixel). Statistical analysis was carried out by one-way ANOVA (***P ≤ 0.001). Bar 50 μm