Table 2.
Specification of comparative mapping of the Pooideae subfamily according to a “round robin” rule
| Species | B. distachyon | H. vulgare | S. cereale | T. aestivum | Ae. tauschii | Ae. speltoides | T. urartu | ×Triticosecale | F. pratensis | L. perenne |
|---|---|---|---|---|---|---|---|---|---|---|
| No. of rDNA loci | 35S 2 loci; 5S 2 loci | 35S 4 loci; 5S 8 loci | 35S 2 loci; 5S 4 loci | 35S 4 loci; 5S 12 loci | 35S 2 loci; 5S 4 loci | 35S 4 loci; 5S 2 loci | 35S 4 loci; 5S 4 loci | 35S 6 loci; 5S 10 loci | 35S 2 loci; 5S 2 loci | 35S 7 loci; 5S 2 loci |
| B. distachyon | Centromeric regions + 2 signals of 35S rDNA | 4 signals of 35S rDNA (NOR chromosomes 6 and 7) | Telomeric regions + 2 signals of 35S + 4 signals of 5S rDNA | Telomeric regions + 4 signals of 35S rDNA | Telomeric regions + 2 signals of 35S rDNA | 4 signals of 35S + 2 signals of 5S rDNA, centromeric regions | Telomeric regions + 4 signals of 35S rDNA | Telomeric regions + 6 signals of 35S + 10 signals of 5S rDNA | Telomeric regions + 2 signals of 35S rDNA | Telomeric regions + 7 signals of 35S rDNA |
| H. vulgare | 35S rDNA | Chromosome labeling + centromeric regions | Chromosome labeling + 2 pericentromeric signals in chromosomes without rDNA loci | Chromosome labeling, in some chromosomes lack of signals in centromeric and terminal regions | Labeling of chromosome segments | Chromosome labeling + pericentromeric signals | Chromosome labeling + 2 pericentromeric signals | Pericentromeric signals in 14 chromosomes of B genome | Chromosome labeling + 2 signals of 35S rDNA | Chromosome labeling + 7 signals of 35S rDNA |
| S. cereale | Strong dispersed signals + 2 signals of 35S + 2 signals of 5S rDNA | Centromeric regions | Chromosome labeling + telomeric regions | Chromosome labeling and signals in chromosomes without rDNA loci | Chromosome labeling + 6 terminal signals in chromosomes without rDNA loci | Chromosome labeling without terminal regions, 4–5 signals in chromosomes without rDNA loci | Chromosome labeling | Labeling of 14 chromosomes (genome R) | Weak chromosome labeling + 2 signals of 35S rDNA | Strong, dispersed signals |
| T. aestivum | 35S rDNA + 5S rDNA + telomeric regions + centromeric regions | Pericentromeric regions | Very weak chromosome labeling without NORs and telomeric regions | Chromosome labeling | Chromosome labeling | Chromosome labeling without terminal regions + pericentromeric signals | Chromosome labeling without terminal regions (1 arm in 2 chromosomes) | Labeling of 28 chromosomes (genomes A and B), very weak labeling of 14 R-genome chromosomes | Chromosome labeling + 2 signals of 35S rDNA | 7 signals of 35S rDNA |
| Ae. tauschii | 35S rDNA + telomeric regions + centromeric regions | Chromosome labeling | Chromosome labeling without telomeric regions | Chromosome labeling | Chromosome labeling + telomeric regions + signals in chromosomes without rDNA loci | Chromosome labeling without terminal regions | Chromosome labeling (8 chromosomes with stronger intensity) | Chromosome labeling, 14 chromosomes with stronger intensity (genome B) | Weak chromosome labeling + 2 signals of 35S rDNA | Chromosome labeling + 5 signals of 35S rDNA |
| Ae. speltoides | 2 signals of 35S + 2 signals of 5S rDNA + centromeric regions | Pericentromeric regions + centromeric regions | Chromosome labeling without terminal regions | 14 signals of 5S rDNA, centromeric regions in 14 chromosomes of B genome | Chromosome labeling + 2 terminal signals in 1 pair without rDNA loci | Chromosome labeling + pericentromeric regions | Chromosome labeling without terminal regions in 1 pair | Labeling of 14 chromosomes (genome B) | Chromosome labeling + 2 signals of 35S rDNA | Weak chromosome labeling + 7 signals of 35S rDNA |
| T. urartu | 2 signals of 35S rDNA | Chromosome labeling without terminal regions | Chromosome labeling without terminal regions | Chromosome labeling, 14 chromosomes with stronger intensity (genome A) | Chromosome labeling without terminal regions | Chromosome labeling without terminal regions | Chromosome labeling | Chromosome labeling (A genome - strong signals, B - weaker intensity, R - very weak labeling) | Weak chromosome labeling + 2 signals of 35S rDNA | Weak chromosome labeling + 7 signals of 35S rDNA |
| ×Triticosecale | 35S rDNA + 5S rDNA + strong dispersed signals | Pericentromeric regions | Chromosome labeling without 2 NORs + telomeric regions | Chromosome labeling without terminal regions, centromeric regions and 2 NORs | Chromosome labeling without terminal regions and NORs | Chromosome labeling of selected regions + pericentromeric signals | Chromosome labeling (6 chromosomes with stronger intensity; 2 chromosomes without labeling of terminal regions) | Chromosome labeling | 2 signals of 35S rDNA | Chromosome labeling |
| F. pratensis | 35S rDNA + centromeric regions | Chromosome labeling + centromeric regions | Chromosome labeling without terminal regions | Weak dispersed signals | Dispersed signals + telomeric regions (not in all chromosomes) | Chromosome labeling without terminal regions and interstitial block in chromosome 5 | Chromosome labeling | Chromosome labeling | Chromosome labeling | Chromosome labeling |
| L. perenne | 2 signals of 35S + 2 signals of 5S rDNA + telomeric regions | Chromosome labeling + 4 signals of 35S rDNA | Chromosome labeling without terminal regions + 2 signals of 35S rDNA | 4 signals of 35S rDNA + telomeric regions | 2 signals of 35S + 2 signals of 5S rDNA + telomeric regions + weak dispersed signals | 4 signals of 35S + 2 signals of 5S rDNA | 4 signals of 35S rDNA + terminal signals in 2 chromosomes + dispersed signals | Chromosome labeling + 6 signals of 35S rDNA | Strong, distinct signals | Chromosome labeling without terminal regions in 1 pair |
Columns = chromosome preparations; rows = gDNA probes