Table 2.
Primers used for the amplification and pyrosequencing of the CYP2B6 and CYP2C9 genes
| Direction | Primer name | Sequence | Product length | |
|---|---|---|---|---|
| Amplification | Forward (*) | CYP2B6_Q172Hf | 5′-CCTGCTGCTTCTTCCTAGGG-3′ | 83 bp |
| Reverse | CYP2B6_Q172Hr | 5′-GACGATGGAGCAGATGATGTTG-3′ | ||
| Forward (*) | CYP2C9_I359Lf | 5′-ATGCAAGACAGGAGCCACATG-3′ | 181 bp | |
| Reverse | CYP2C9_I359Lr | 5′-GGGACTTCGAAAACATGGAGTTG-3′ | ||
| Pyrosequencing | Reverse | CYP2B6_Q172Hseq | 5′-TGATGTTGGCGGTAAT-3′ | |
| Reverse | CYP2C9_I359Lseq | 5′-TGGGGAGAAGGTCAA-3′ |
(*) = primers labelled with biotin