FIG 5.

Influence of gp79 on the induction of the lytic life cycle of ϕCh1. The wild-type strain N. magadii L11 bearing pNB102 (circles; WT) and the ORF79-overexpressing strain N. magadii L11 bearing pNB102-ORF79 (squares; OE) were grown in rich medium. (A) Optical density at 600 nm was measured throughout growth at indicated time points. (B) The virus titer in the culture supernatant 115 h after inoculation was determined by infecting the cured strain N. magadii L13. (C to E) Total protein samples at the same time point were used for Western blot analysis using polyclonal antibodies against protein E, the tail fiber protein gp34, or the methyltransferase M.NmaϕCh1-I, as indicated. The respective purified proteins were used as positive control (+). (F) A Southern blot analysis with BglII-digested chromosomal DNA using a fragment of the ϕCh1 genome as a probe (generated by PCR with the primers Soj-5 and Soj-3) resulted in the expected signal at 3,130 bp for both strains. A New England BioLabs biotinylated DNA marker (L) was used as a size control.