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Western blot analyses of transfected RK13 cells. Lysates prepared 48 h after transfection with expression plasmids for wild-type gHKa, modified gH32/98, or the empty vector (Mock) were separated by SDS-PAGE. The blot was incubated with a PrV gH-specific rabbit antiserum. Molecular masses (kDa) of marker proteins are indicated.
