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In vitro growth kinetics of PrV mutants. RK13 cells were infected with pPrV-gHK, pPrV-gH32/98K, pPrV-gH32/98KgBB4.1, or pPrV-gH32/98KΔgLZgBB4.1 at an MOI of 0.01 (A) or 5 (B). Cells were harvested together with the supernatants after 0, 6, 12, 18, 24, 36, 48, and 72 h at 37°C and lysed by freeze-thawing, and progeny virus titers were determined on RK13 cells. Shown are mean results of four independent experiments with corresponding standard deviations.
