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Penetration kinetics of PrV mutants. RK13 cells were infected with approximately 250 PFU of pPrV-gHK, pPrV-gH32/98KgBB4.1, or pPrV-gH32/98KΔgLZgBB4.1. After adsorption at 4°C, the cells were incubated for 0, 15, 30, 60, 120, and 240 min at 37°C prior to acid inactivation of nonpenetrated virus. Numbers of plaques formed after 48 h were compared to those obtained without inactivation. Mean percentages of three independent experiments and standard deviations are shown.
