TABLE 1.
Oligonucleotide primers for mutagenesis, PCR, and sequence analyses
| Primer name | Sequence (5′→ 3′)a | Nucleotide positionsa,b |
|---|---|---|
| PGH32/98-F | CTCGCCCGCGGCGCG/CCGCCCGTCTCCGCG | 60848–60862/61061–61075 |
| PGH32/98-R | CGCGGAGACGGGCGG/CGCGCCGCGGGCGAG | 60848–60862/61061–61075 (r) |
| UL1-For | CACAAAGCTTAGGATACACCAGCCGCGATG | 95482–95501 (r) |
| UL1-Rev | CACAGAATTCGGTCTCTTACTCGGCGGGGG | 95008–95027 |
| PgBR-F | ACAGGATCCCTTCGCGCACGACACGC | 19984–20000 (r) |
| PgBR-R | ACAGGATCCTCATCGTGGTCGCGGTC | 16250–16266 |
| gB-AssFW | TAACGGATCCATGCCCGCTGGTGGCGG | 19657–19673 (r) |
| gB-AssRV | CAGAATTCCTACAGGGCGTCGGGGTCC | 16911–16929 |
| gB-P3 | GATCTCCTGCACGGGGACGGGCACG | 19032–19056 (r) |
| gB-P7 | CCATCTACCGGCGGCGCTACAACA | 18329–18352 (r) |
| gD-For | CACAGAATTCACCTGCCAGCGCCATGC | 121129–121148 |
| gD-Rev | CACAGAATTCCATCGACGCCGGTACTGC | 122353–122370 (r) |
| PgH-PSF | TTCACGTCGGAGATGGGG | 60611–60628 |
| PgH-PSF2 | GGAAGCCCTTCGACCAG | 61875–61891 |
| PgHK-PSR | CGCGAGCCCATTTATACCC | (Kanr) |
| PgH-PSR2 | GTCGAGCAGGCTGAAGG | 62055–62071 (r) |
| PgH-WH3 | TGCACGAGAGCGACGACTACC | 61479–61499 |
| PgH-E555AR | CAGGTGCTGGGACGCGGGCATGCGATC | 62420–62446 (r) |
a
Nucleotide positions in the PrV Ka genome refer to GenBank accession number JQ809328 (57). Nonmatching nucleotides are shown in boldface, and restriction sites for cloning of PCR products are underlined.
b
Slashes in the first two primer sets denote the deletion of nucleotide positions 60863 through 61060. Reverse-strand PCR primers are indicated (r). PgHK-PSR binds to the inserted kanamycin resistance gene (Kanr) of the PrV recombinants.