FIG 2.
Mutagenesis analysis of hPIV2 NP Q202 using the Rluc minireplicon assay. (A) Relative Rluc activity in the minireplicon assay with the hPIV2 polymerase complex including NP mutants. The Rluc expression from minigenomes is normalized to internal control Fluc expression, and relative values are shown (NPwt = 1). NP−, result from the Rluc minigenome without NP plasmid. Data represent means and standard deviations from triplicate experiments. Expression of NP, P, and L was detected by Western blotting using specific MAbs. (B) Extension from panel A, displaying the relative Rluc activity in the minireplicon assay using NP Q202T, P, and K. (C) Relative amount of antigenome during the hPIV2 Rluc minireplicon assay. The NP-encapsidated antigenome was obtained by immunoprecipitation after performing the assay. The relative amounts of antigenome were measured by qRT-PCR. Data represent means and standard deviations from triplicate experiments. *, P < 0.01; **, P < 0.05.