FIG 7.

Effects of NP^Q202A on unique minireplicon assays using incomplete minigenomes partially lacking leader sequences and which do not conform to the rule of six. (A) Relative Rluc expression in the minireplicon assay partially lacking the leader sequence determined by using the hPIV2 polymerase complex including NP^wt or NP^Q202A. (B) Relative luciferase expression in the minireplicon assay partially lacking 30 to 35 nucleotides from the 3′ terminus determined by using the hPIV2 polymerase complex including NP^wt or NP^Q202A. The Rluc expression from minigenomes is normalized to an internal control Fluc expression, and relative values are shown (NP^wt = 1). NP−, result for the Rluc minigenome without NP plasmid. “NP wt” in the graph for NP^Q202A indicates the result for NP^wt using the normal minigenome. Data represent means and standard deviations from triplicate experiments. *, P < 0.01.