FIG 7.

Roles of the cytoplasmic tails of HA and NA in protein colocalization. MDCK cells were infected with recombinant influenza virus encoding HA and NA lacking their cytoplasmic tails. (A) The distributions of the cytoplasmic tail-less glycoproteins (12-nm gold particles) were compared to that of M2 (6-nm gold particles). Transfected 293T cells expressing M2 and the cytoplasmic tail deletion mutants of either HA or NA were also examined (n = 2 for M2-HAtail− in infected cells, n = 2 for M2-HAtail− in transfected cells, n = 2 for M2-NAtail− in infected cells, and n = 2 for M2-NAtail− in transfected cells). (B) Spatial localization patterns of M1 (6-nm gold particles) were compared to those of the cytoplasmic tail-minus mutants of HA and NA (12-nm gold particles) in virus-infected MDCK cells and separately in transfected 293T cells by a bivariate Ripley analysis (n = 2 for M1-HAtail− in infected cells, n = 3 for M1-HAtail− in transfected cells, n = 4 for M1-NAtail− in infected cells, and n = 2 for M1-NAtail− in transfected cells).