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. 2017 Apr 13;91(9):e02250-16. doi: 10.1128/JVI.02250-16

FIG 6.

FIG 6

NEAT1 upregulation is dependent on live viral replication. (A) HEK293 cells were transfected with pcDNA3.1 (vector), pcDNA3.1-NP, pcDNA3.1-G1, or pcDNA3.1-G2 for 48 h, and NEAT1 levels were tested by qRT-PCR. Values are means ± SD (*, P < 0.01; **, P < 0.001; one-way ANOVA). (B) HUVECs were mock infected or HTNV infected with polyclonal sheep antibodies against IFN-α and IFN-β (1:100 dilution; anti-IFN-I), and the cells were collected for NEAT1 detection by qRT-PCR at different times postinfection. Values are means ± SD (*, P < 0.01; **, P < 0.001; Student's t test, compared with results at 0 dpi). (C, D, and E) HUVECs were stimulated with IFN-α (C), IFN-β (D), or IFN-γ (E) at various doses for 12 h; qRT-PCR was then performed to assess NEAT1 expression levels. Values are means ± SD (*, P < 0.01; **, P < 0.001; one-way ANOVA). (F and G) HUVECs were stimulated with IL-1β (F) or TNF-α (G) at various doses for 12 h, and then qRT-PCR was performed to assess NEAT1 expression levels. Values are means ± SD (*, P < 0.01; **, P < 0.001; one-way ANOVA). The experiments were performed independently at least three times with similar results.