FIG 3.
BMRF1 functions as an activator to turn on a subset of EBV late gene promoters in HEK293T cells. HEK293T cells (1 × 105 cells) were seeded into a 12-well culture plate and cotransfected with the doses of BMRF1 or BMRF1ΔTA indicated, with or without Rta-expressing plasmids and with the reporter plasmids pGL2-BcLF1 (A and F), pGL2-BLLF1 (B and G), pGL2-BLLF2 (C and H), pGL2-BDLF3 (D and I), and pGL2-BILF2 (E and J) by BBS for 48 h. In all reporter assays, the Renilla Luc plasmid (pRL-null; 250 ng/well) was cotransfected as a transfection control. The luciferase activities were detected by a luminometer using a Dual-Glo Luciferase Assay kit. The promoter upstream region of the indicated gene construct in the reporter plasmid was described in Materials and Methods. Representative data of three independent experiments are presented.