FIG 7.

Knockdown of BRG1 reduced the transactivation activities of BMRF1 on the promoters of BHLF1, BLLF1, and BcLF1 in reporter assays in HEK293T cells and reduced the expression of late proteins BLLF1 and BcLF1 in Rta-reactivated NA cells. (A to D) HEK293T cells were transduced with shLuc or shBRG1 lentivirus for 48 h and selected with puromycin for 5 days. The shLuc control and BRG1 knockdown cells were cotransfected with the indicated effector and reporter plasmids pGL2-BHLF1, pGL2-BLLF1, pGL2-BcLF1, and pGL2-BDLF3 by the calcium phosphate-BES method. At 48 h posttransfection, luciferase activities and protein expression were detected by reporter assay and Western blotting. Representative data of two independent experiments are shown. (E to G) The shLuc control and BRG1 knockdown NA cells were transfected with a vector control or Rta-expressing plasmid for 48 h. The transfected cells and culture supernatant were harvested for detection of lytic proteins (E), intracellular EBV DNA copy number (F), and extracellular virion production (G). Representative data of two independent experiments are presented.