FIG 8.

BMRF1 and BRG1 bind to the viral late gene promoters BcLF1p and BLLF1p. HEK293T cells were transfected with a plasmid expressing pGL2-basic Luc or serially deleted BcLF1 promoter (A) or BLLF1 promoter (B) Luc-expressing plasmids coupled with a vector control or HA-BMRF1-expressing plasmid for 48 h. Luciferase activities were detected using a Dual-Glo Luciferase Assay kit. Representative data of two independent experiments are presented. (C to F) At 36 h postinduction, NA cells (1 × 10⁷ cells) (Cand D) and Akata EBV-positive cells (2 × 10⁷ cells) (E and F) were harvested for ChIP-qPCR analysis with antibody against BMRF1, BRG1, or control IgG on promoter regions of BLLF1, BcLF1, BDLF3, and BILF2. To normalize the ChIP-qPCR data, the signals obtained from the ChIP assay were divided by the signals obtained from an input sample, and the data are presented as the percentage of input. Representative data of two independent experiments are presented.