TABLE 2.
List of the primers used in this study
| Primer function and name | Forward primer sequence (5′–3′) | Reverse primer sequence (5′–3′) |
|---|---|---|
| Plasmid and bacmid construction | ||
| LMRC439/440 | CCGGGATCCATGGAAACCACTCAGACTCT | CGCGAATTCTTAAATGAGGGGGTTAAAGGC |
| LMRC718/719 | TCTGCTCTGGTACGTTGGCTTCTGCTGCTGCTTGTGATCTGTAGGCTGGAGCTGCTTC | CCGTACTGGCGGCCGCCTCTTCGGAGGCGTGGTTAAATAATTCCGGGGATCCGTCGACC |
| LMRC772 | TGGCTGTTCAGCTCGCGTCGGAGGCCAGGCAGAA | |
| LMRC912 | TGGTACCGAGCTCGGATCCATGTACCCATACGATGTTCCAGATTACGCTATGGAAACCACTCAGACTC | |
| LMRC955/956 | ATCGGTACCTTTCTGGTGCATTTGCGAGC | CTGAGATCTCTGGTGGACACATGATGTGT |
| LMRC1000/999 | TGGGTACCTTCGTACCCTTCTGGGCCGG | GCTCTAGAGGGAATTCCGGAATCTCAAC |
| LMRC1001/999 | TGGGTACCTCTCCGACGATCTCTAATGG | GCTCTAGAGGGAATTCCGGAATCTCAAC |
| LMRC1003/1002 | TGGGTACCTTCGGATAACGCCGAGTAGA | GCTCTAGAGACACAAGGTAAGAGGGAGATGG |
| LMRC1004/1002 | TGGGTACCGATGATAAGGCGAAACATTG | GCTCTAGAGACACAAGGTAAGAGGGAGATGG |
| RT-PCR and RT-qPCR | ||
| BcLF1 | GTTGAGGCTGTTTAGGGTATG | CAATCCCAAGTACACGACC |
| BLLF1 | TACTGGGGTGGGACTTGTT | GAGCGGGGGAGATTACTG |
| BLLF2 | TGACCAGCAGCAGCAGAAG | ATGTGTCCACCAGTTCGCC |
| BILF2 | ACTGGTGCTTGTGGTGTG | CTGTGTGGGGTCTGTTTGT |
| BSRF1 | GGCGGGCTAAACAGAACGA | AAGGCGGAGTTGATGAAAG |
| BaRF1 | TCCTTCTACAGCATAGCCCT | AGGTCATCTACCACCAGCAT |
| BORF2 | GGACACCCACCACACAGCA | TCAAACTCCTCCCCGTAGA |
| EBV genome copy number detection | ||
| BamHI W | CCCTGGTATAAAGTGGTCCT | AAGTCCACTTACCTCTGG |
| HBG | GCTTCTGACACAACTGTGTTCACTAGC | CACCAACTTCATCCACGTTCACC |
| ChIP-qPCR | ||
| BcLF1p | ATGAATGACTGCCAGGAGCT | GGATGATAAGGCGAAACATTG |
| BILF2p | ATTCACTCTAGCACCTGC | GCTGTTGTTGCAGAATATGA |
| BLLF1p | AATCCAGCTTCTCGTAGAGAT | AAAGGTCACGTGACACCTAC |
| BDLF3p | CGCGTCTGGACGCAGA | CGGAAGCAATGTCGTGTTG |