FIG 5.

Determinants in the GP2 subunits account for the reduced EBOV2014-GP-mediated entry into nonhuman primate cells. Rhabdoviral pseudotypes harboring no glycoprotein (pCAGGS, negative control, white), VSV-G (positive control, gray), EBOV1976-GP wt (blue), EBOV2014-GP (red), and the indicated chimeras were inoculated onto different mammalian cell lines. At 18 h postinoculation, the transduction efficiency was quantified by measuring the firefly luciferase activity in cell lysates. The averages of three independent experiments (with separate pseudotype preparations) are shown. Results were normalized against transduction mediated by EBOV1976-GP wt (set as one) and are shown as x-fold changes. Error bars indicate the standard errors of the mean. A paired, two-tailed Student t test was used to assess statistical significance (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, no significance).