FIG 3.

Electron microscopic analysis of the effects of C1 on the morphology of the HIV-1 core. HIV-1 particles were produced in the presence or absence of C1 (40 μM) and analyzed by thin-section electron microscopy. Representative images of mature, eccentric, and immature particles are shown in the two left, middle, and right pairs of images, respectively. Fractions of mature, immature, and eccentric particles were quantified and are shown in the right panel. Results are percentages normalized for the different morphologies from counting minimally 100 particles per experiment; error bars show the range in values across two independent experiments. The ALLINI BI-D (0.18 μM) was included in the experiment as a known compound that perturbs HIV-1 maturation (38).