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C1 does not inhibit HIV-1 particle encapsidation of viral RNA. RNA was extracted from DNase-treated HIV-1 particles produced in the presence or absence of C1 (40 μM) and analyzed by quantitative RT-PCR using primers specific for HIV-1 sequences. As controls, the RNA samples were subjected to PCR analysis without prior reverse transcription (no RT). Results shown are averages from three independent experiments, with error bars representing standard deviations.
