FIG 7.

R132T substitution in CA renders HIV-1 resistant to C1. (A) Cultures of CEM-SS cells were inoculated with HIV-1 (top) and HIV-1.CA.R132T (bottom), and cells were cultured in the presence of the indicated concentrations of C1. HIV-1 replication was monitored by quantifying p24 in the culture supernatants; the results are representative of those observed in two independent experiments. (B) Wild-type and R132T CA mutant GFP reporter viruses were produced in the presence and absence of C1 and assayed for infectivity by flow cytometry for GFP expression following infection of target cells in the absence of added inhibitor. Shown are the mean values from two independent experiments. (C) Quantification of wild-type and R132T mutant particle morphologies in the presence of DMSO or 40 μM C1 (means ± standard deviations for n = 2 experiments, with 65 to 424 particles counted per experiment). dpi, days postinfection.