FIG 8.

C1 induces aberrant cross-links in mutant particles capable of forming CA-CA disulfide bonds at the interhexamer interface. HIV-1 particles were produced by transfection of HIV-1 proviral constructs encoding the indicated Cys substitutions and cultured in the presence or absence of C1 (100 μM). Culture supernatants were harvested 2 days after transfection, and virions were pelleted and analyzed by nonreducing SDS-PAGE. The gel was immunoblotted with a probe for CA. Shown are representative results from one of three independent experiments. Asterisks indicate high-molecular-mass species that either formed or were enhanced by the presence of C1 and were dependent on Arg132 in CA.