FIG 4.

Serum neutralizing antibody responses elicited by anti-LOX-1.Env gp140 and anti-CD40.Env gp140 fusion protein administration. (A) Neutralizing antibodies against HIV-1 were measured as a function of reduction in Tat-regulated reporter gene expression in TZM-bl cells infected with HIV-1 isolate MW965. Neutralizing antibody titer values are ID₅₀s. Samples were tested from sera collected at weeks −4, 14, 26, and 32. Groups are indicated beside the graph. Each dot represents the value for one individual macaque, responders in red, nonresponders in blue. (B) The graph shows the area under MB curves (see Materials and Methods) used to summarize overall breadth and magnitude of neutralizing antibody activities against A3R5 virus for each individual time point. Samples were tested from sera collected at weeks −4, 14, 26, and 32. Groups are indicated below the graph. The midline of the box indicates the median, and the ends of the box indicate the 25th and 75th percentiles. The whiskers that extend from the top and bottom of the box extend to the most extreme data points that are no more than 1.5 times the interquartile range (i.e., the height of the box) or if no value meets this criterion, to the data extremes. Table 4 shows analysis of paired comparisons of ID₅₀ values for isolates MW965 and Th023.6.