Table 1. Binding Affinity K_D ≡ k^off/k^on Determined With DCS (center column) and MST (right column) for the Three Proteins Used in This Work^a.

protein	KD [μM] (DCS)	KD [μM] (MST)
HSA	2.8 ± 0.2	2.4 ± 0.6
Tf	0.65 ± 0.08	1.8 ± 0.4
Fib	(11 ± 2) × 10–3	(2.2 ± 0.9) × 10–3

^aThe values for HSA coincide within the error bars and are consistent with previous literature.²⁴⁻²⁶ Those for Tf and Fib agree only in order of magnitude, however the MST measurements are possibly biased by agglomerates of NPs²⁷ and are based on less data and with larger noise (Figure 1) than the DCS measures. K_D is expressed in μM = 10^–6 M.