Figure 3.

ERdj3 is up-regulated during ER stress. (A) HepG2 cells were treated for 6 h in the absence (-) or presence of tunicamycin (Tm) or thapsigargin (Tg). RNA was extracted and subjected to Northern blotting with the indicated probes. (B) HeLa cells were treated as described above and then pulse-labeled for 30 min. Cell lysates were immunoprecipitated with anti-ERdj3 antiserum followed by protein A-Sepharose (PA) as indicated and samples were subjected to SDS-PAGE.