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ERdj3 interacts with three unfolded substrates. COS-1 cells were cotransfected with human Igγ heavy chain (A), NS-1 nonsecreted κ light chain (B), and VSV-G ts045 (C) along with the indicated cDNAs. Cells were metabolically labeled for 2 h (VSV-G transfectants were incubated at 39°C for 1 h before labeling at 39°C for 30 min) and then incubated with DSP. Cell lysates were prepared and immunoprecipitated with the indicated antisera and analyzed by reducing SDS-PAGE.
