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. 2005 Jan;16(1):40–50. doi: 10.1091/mbc.E04-05-0434

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Figure 8. — ERdj3 interacts with light chain intermediates transiently, whereas BiP shows prolonged binding. COS-1 cells were transiently transfected with the indicated cDNAs and labeled as in Figure 7. Media from each of the various time points were immunoprecipitated with anti-λ antiserum (A). The cell lysates were divided and immunoprecipitated with antisera specific for mouse λ (B), rodent BiP (C), or HA-tagged ERdj3 (HA/J3) (D) and analyzed by nonreducing SDS-PAGE. Nearly half of the sample for lane 10 in D was lost.