Table 1.

Protocols for pre-treatment of Toxoplasma gondii-spiked milk samples.

Aliquot (n)	Contamination rate (tachyzoites/10 mL)	Protocol	Time needed
1	105	I	1 h
2	106
3	105	II	20 min
4	106
5	105	III	24 h
6	106

I, no pre-treatment; II, anti-casein treatment; III, overnight incubation at 56°C.