Table 3.

Restriction enzymes (as described by Su et al., 2010) for the polymerase chain reaction-restriction fragment length polymorphism protocol.

Genetic marker	Restriction enzymes	Temperature (°C)	Buffer	Cutting site
SAG1	Sau96I	37	Tango	5’ G↓GNCC 3’ 3’CCNG↑G5’
	HaeIII	37	Tango	5´ -GG↓CC-3´ 3´ -CC↑GG-5´
SAG2	HinfI	37	Taq	5’G↓ANTC3’ 3’CTNA↑G5’
	TaqI	65	Taq	5’T↓CGA3’ 3’AGC↑T 5’
SAG3	NciI	37	Tango	5’ CC↓SGG 3’ 3’GGS↑CC 5’
GRA6	MseI	65	R	5’T↓TAA3’ 3’AAT↑T5’
L358	HaeIII	37	R	5’GG↓CC3’ 3’CC↑GG5’
	HinII	37	R	5’CATG↓3’ 3’↑GTAC5’
PK1	RsaI	37	Tango	5’GT↓AC3’ 3’CA↑TG5’
	Eco88I	37	Tango	5’ACGT↓3’ 3’↑TGCA5’
C29-2	RsaI	37	Tango	5’GT↓AC3’ 3’CA↑TG5’
	MaeII	65	Tango	5’ACGT3’ 3’TGCA5’
BTUB	Bsh1258I	37	Taq	5’CGRY↓CG3’ 3’GC↑YRGC5’
	TaqI	65	Taq	5’T↓CGA3’ 3’AGC↑T 5’
C22-8	Alw26I	37	Tango	5’GTCTCN1↓3’ 3’CAGAGN5↑5’
	MboII	37	Tango	5’GAAGAN8↓3’ 3’CTTCTN7↑5’’
APICO	AfiII	37	Tango	5’C↓TTAAG3’ 3’GAATT↑C5’
	DdeI	37	Tango	5’C↓TNAG3’ 3’GANT↑C5’