Figure 6.

R-Ras enhances Rho activity and decreases Rac activity. T47D cells were plated on a collagen-coated plate (3 μg/ml) for 45 min. (A) Cells were lysed and 30 μg PBD:GST was incubated with the lysates to pull down active Rac. Active and total protein was detected by Western blotting. Expression of activated R-Ras (38V) decreases (p = 0.15) Rac activity, whereas expression of dominant negative Rac slightly enhances (p = 0.72) Rac activity. (B) RBD:GST was used to determine Rho activity. Expression of activated R-Ras (38V) significantly enhances Rho activity, whereas expression of dominant negative R-Ras (41A) decreases (p = 0.08) Rho activity. Quantification was performed on three individual experiments and is shown in the bar graphs on the right (±SEM; ^*p < 0.05 vs. Control, + collagen).