Figure 7.

R-Ras alters the spatial localization of active Rac and Rho. After cell fixation and permeabilization, 100 μg/ml GBD: GST was incubated with the cells, followed by an anti-GST antibody, and then secondary antibody. Expression of activated R-Ras (38V) caused an increase in active Rho along the periphery of cells and a loss of active Rac (see arrows). Expression of dominant negative R-Ras (41A) caused an increase in the amount of activated Rac localized to lamellipodia (see arrows). Scale bar, 25 μm.