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Effects of double CRK knockdowns on the growth of procyclic-form T. brucei cells. Strain 29-13 cells harboring each double-CRK-RNAi construct were incubated at 26°C in culture medium without (-Tet) or with (+Tet) 1.0 μg/ml tetracycline. The numbers of cells were monitored daily. The insets show the intracellular mRNA levels after 3 d of RNAi as monitored by semiquantitative RT-PCR. Quantitation of α-tubulin mRNA (TUB) was included as a control.
