Fig. 2.

PvRxLR16 effector domains function in plant nucleus. a Green fluorescence-tagged PvRxLR16 were transiently expressed in Arabidopsis protoplasts via PEG-mediated transformation approach. Scale bar =25 μm. b GFP- PvRxLR16 fusion protein was expressed in onion epidermal cells. Scale bar =50 μm. c Nuclear localization is required for PvRxLR16-triggering cell death. N. benthamiana leaves were agroinfiltrated with the indicated constructs 2 d and 5 d before assessment of GFP confocal imaging and cell death observation, respectively. NES and nes represent the nuclear export signal and nonfunctional NES. Scale bar =20 μm. d Quantification of cell death by measuring electrolyte leakage. Error bars represent standard errors from three biological replicates (^** for P < 0.01 and ^* for P < 0.05, Dunnett’s test)