Fig. 5.

SGT1, Hsp90, and RAR1 were required for PvRxLR16-induced cell death in N. benthamiana. a PvRxLR16 was transiently expressed in N. benthamiana leaves silenced for pTV00 (control), SGT1, Hsp90, RAR1, and SERK3. GFP and INF1 are control proteins. Typical symptoms were photographed after 5 d after agroinfiltration. b Quantification of cell death by measuring electrolyte leakage. Averages and standard errors were calculated from three independent experimental treatments (^** for P < 0.01 and ^* for P < 0.05, Dunnett’s test). c Transcript levels of indicated genes in silenced N. benthamiana measured by quantitative RT-PCR. Error bars represent standard errors from three biological replicates (^**, P < 0.01, Dunnett’s test)