Figure 7.

Selective reduction of vesicular zinc in the absence of neuronal AP-3. Histochemically reactive zinc was assessed by Timm's staining of control (A), Ap3b1^-/- (B), Ap3b2^-/- (C), and mocha (D) coronal brain sections. (A) Values 1 and 2 correspond to distinct layers of the brain cortex, and 3 and 4 correspond to the stratum oriens and radiatum of the hippocampus. Mocha possesses prominently reduced Timm's stain. Ap3b2^-/- hippocampus and cortex have reduced Timm's deposits. Note the increase in Ap3b1^-/- cortex and stratum radiatum. (E) Quantitative analysis of Timm's deposits. Control sections were assigned a 0 value. Increases are seen as values >0, decreased Timm's staining correspond to values <0. Asterisks mark statistically significant differences (at least p < 0.05). The n values are described in Materials and Methods.